3RMZ

Crystal Structure of the W199F-MauG/pre-Methylamine Dehydrogenase Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Methylamine utilization protein MauG	polymer	373	41107.6	2	UniProt (Q51658) Pfam (PF03150) In PDB	Paracoccus denitrificans
2	C, E	Methylamine dehydrogenase light chain	polymer	137	15025.6	2	UniProt (A1BBA0) Pfam (PF02975) UniProt (by SIFTS) (P22619) In PDB	Paracoccus denitrificans
3	D, F	Methylamine dehydrogenase heavy chain	polymer	386	42449.3	2	UniProt (A1BB97) Pfam (PF06433) In PDB	Paracoccus denitrificans
4	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	A, B	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	A, B	HEME C	non-polymer		618.5	4	Chemie (HEC)
7	A, B	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
8	D	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
9	D	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
10	F	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
11	F	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
12	F	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
13		water	water		18.0	1876	Chemie (HOH)

Sequence modifications

A, B: 1 - 367 (UniProt: Q51658)

PDB	External Database	Details
Phe 199	Trp 219	engineered mutation
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

C, E: 1 - 131 (UniProt: A1BBA0)

PDB	External Database	Details
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	197164.9
Non-Polymers*	Number of molecules	17
	Total formula weight	3607.2
All*	Total formula weight	200772.2

*Water molecules are not included.