3RFA

X-ray structure of RlmN from Escherichia coli in complex with S-adenosylmethionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribosomal RNA large subunit methyltransferase N	polymer	404	45328.8	2	UniProt (P36979) Pfam (PF21016) Pfam (PF04055)	Escherichia coli	23S rRNA m2A2503 methyltransferase
2	C, E (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D, F (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
4	G, H (A, B)	water	water		18.0	181	Chemie (HOH)

Sequence modifications

A, B: 1 - 384 (UniProt: P36979)

PDB	External Database	Details
Gly 385	-	expression tag
Asn 386	-	expression tag
Ser 387	-	expression tag
Ser 388	-	expression tag
Ser 389	-	expression tag
Val 390	-	expression tag
Asp 391	-	expression tag
Lys 392	-	expression tag
Leu 393	-	expression tag
Ala 394	-	expression tag
Ala 395	-	expression tag
Ala 396	-	expression tag
Leu 397	-	expression tag
Glu 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90657.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1500.2
All*	Total formula weight	92157.8

*Water molecules are not included.