3RFA

X-ray structure of RlmN from Escherichia coli in complex with S-adenosylmethionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribosomal RNA large subunit methyltransferase N	polymer	404	45328.8	2	UniProt (P36979) Pfam (PF21016) Pfam (PF04055)	Escherichia coli	23S rRNA m2A2503 methyltransferase
2	C, E (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D, F (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
4	G, H (A, B)	water	water		18.0	181	Chemie (HOH)

Sequence modifications

A, B: 1 - 384 (UniProt: P36979)

PDB	External Database	Details
Gly 385	-	expression tag
Asn 386	-	expression tag
Ser 387	-	expression tag
Ser 388	-	expression tag
Ser 389	-	expression tag
Val 390	-	expression tag
Asp 391	-	expression tag
Lys 392	-	expression tag
Leu 393	-	expression tag
Ala 394	-	expression tag
Ala 395	-	expression tag
Ala 396	-	expression tag
Leu 397	-	expression tag
Glu 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90657.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1500.2
All*	Total formula weight	92157.8

*Water molecules are not included.