3RDM

Crystal structure of R7-2 streptavidin complexed with biotin/PEG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Streptavidin	polymer	153	15966.4	1	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
3	A	BIOTIN	non-polymer		244.3	1	Chemie (BTN)
4		water	water		18.0	127	Chemie (HOH)

Sequence modifications

A: 26 - 153 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Ile 14	-	expression tag
Glu 15	-	expression tag
Gly 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Arg 19	-	expression tag
Leu 20	-	expression tag
Ile 21	-	expression tag
Lys 22	-	expression tag
His 23	-	expression tag
Met 24	-	expression tag
Thr 25	-	expression tag
Leu 42	Phe 53	engineered mutation
Gly 65	Ser 76	engineered mutation
Ser 66	Arg 77	engineered mutation
Ser 103	Thr 114	engineered mutation
Val 121	Trp 132	engineered mutation
Thr 123	Leu 134	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	15966.4
Non-Polymers*	Number of molecules	2
	Total formula weight	482.6
All*	Total formula weight	16449.0

*Water molecules are not included.