3R84

Structure of the Mediator head subcomplex Med11/22

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Mediator of RNA polymerase II transcription subunit 11	polymer	86	10129.4	12	UniProt (Q99278) Pfam (PF10280)	Saccharomyces cerevisiae (yeast)	Mediator complex subunit 11
2	B, D, F, H, J... (B, D, F, H, J...)	Mediator of RNA polymerase II transcription subunit 22	polymer	92	10586.4	12	UniProt (P32570) Pfam (PF06179)	Saccharomyces cerevisiae (yeast)	Mediator complex subunit 22, Suppressor of RNA polymerase B 6
3	AA, BA, CA, DA, EA... (C, D, E, F, G...)	water	water		18.0	964	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O, Q, S, U, W: 5 - 89 (UniProt: Q99278)

PDB	External Database	Details
Gly 4	-	expression tag

B, D, F, H, J, L, N, P, R, T, V, X: 2 - 89 (UniProt: P32570)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Mse 1	-	expression tag
Ile 27	Met 27	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	248589.3
All*	Total formula weight	248589.3

*Water molecules are not included.