3R24

Crystal structure of nsp10/nsp16 complex of SARS coronavirus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyl transferase	polymer	344	38610.0	1	UniProt (P0C6X7) Pfam (PF06460)	SARS coronavirus (SARS-CoV)	nsp16
2	B (B)	Non-structural protein 10 and Non-structural protein 11	polymer	155	16789.1	1	UniProt (P0C6U8) Pfam (PF09401)	SARS coronavirus (SARS-CoV)	nsp10, Growth factor-like peptide, GFL, nsp11
3	C (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
4	D, E (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	F, G (A, B)	water	water		18.0	467	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0C6X7)

PDB	External Database	Details
Met -45	-	expression tag
His -44	-	expression tag
His -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
Ser -38	-	expression tag
Ser -37	-	expression tag
Gly -36	-	expression tag
Leu -35	-	expression tag
Val -34	-	expression tag
Pro -33	-	expression tag
Arg -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
Gly -29	-	expression tag
Met -28	-	expression tag
Lys -27	-	expression tag
Glu -26	-	expression tag
Thr -25	-	expression tag
Ala -24	-	expression tag
Ala -23	-	expression tag
Ala -22	-	expression tag
Lys -21	-	expression tag
Phe -20	-	expression tag
Glu -19	-	expression tag
Arg -18	-	expression tag
Gln -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Asp -14	-	expression tag
Ser -13	-	expression tag
Pro -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

B: 10 - 152 (UniProt: P0C6U8)

PDB	External Database	Details
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asp 1	-	expression tag
Lys 2	-	expression tag
Ile 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55399.1
Non-Polymers*	Number of molecules	3
	Total formula weight	529.3
All*	Total formula weight	55928.4

*Water molecules are not included.