3R1H

Crystal structure of the Class I ligase ribozyme-substrate preligation complex, C47U mutant, Ca2+ bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	U1 small nuclear ribonucleoprotein A	polymer	98	11340.3	2	UniProt (P09012) Pfam (PF00076)	Homo sapiens (human)	U1A, U1 snRNP A, U1-A
2	B, E (B, E)	5'-R(*UP*CP*CP*AP*GP*UP*A)-3'	polymer	7	2181.4	2
3	C, F (C, F)	Class I ligase ribozyme	polymer	130	42102.9	2
4	AA, BA, CA, DA, EA... (F, C, E)	CALCIUM ION	non-polymer		40.1	26	Chemie (CA)
5	GA, HA (C, F)	water	water		18.0	39	Chemie (HOH)

Sequence modifications

A, D: 1 - 98 (UniProt: P09012)

PDB	External Database	Details
His 31	Tyr 31	engineered mutation
Arg 36	Gln 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	111249.2
Non-Polymers*	Number of molecules	26
	Total formula weight	1042.0
All*	Total formula weight	112291.2

*Water molecules are not included.