3R07

Structural analysis of an archaeal lipoylation system. A bi-partite lipoate protein ligase and its E2 lipoyl domain from Thermoplasma acidophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lipoate-protein ligase A subunit 1	polymer	285	32695.1	1	UniProt (Q9HKT1) Pfam (PF21948)	Thermoplasma acidophilum DSM 1728	Lipoate--protein ligase subunit 1
2	B (C)	Putative lipoate-protein ligase A subunit 2	polymer	91	10561.2	1	UniProt (Q9HKT2) Pfam (PF10437)	Thermoplasma acidophilum DSM 1728	Lipoate--protein ligase subunit 2
3	C, D (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
4	E, F (A, C)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5	G, H (A, C)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A: 1 - 262 (UniProt: Q9HKT1)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

C: 1 - 88 (UniProt: Q9HKT2)

PDB	External Database	Details
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43256.4
Non-Polymers*	Number of molecules	4
	Total formula weight	354.4
All*	Total formula weight	43610.8

*Water molecules are not included.