3QZU
Crystal structure of Bacillus subtilis Lipase A 7-fold mutant; the outcome of directed evolution towards thermostability
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Lipase estA | polymer | 181 | 19266.5 | 2 | UniProt (P37957) Pfam (PF01674) | Bacillus subtilis subsp. subtilis | Lipase A, Triacylglycerol lipase |
| 2 | C, H (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | D, I (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E, F, G, J, K (A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | L, M (A, B) | water | water | 18.0 | 482 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 181 (UniProt: P37957)
| PDB | External Database | Details |
|---|---|---|
| Gln 33 | Arg 64 | engineered mutation |
| Asn 34 | Asp 65 | engineered mutation |
| Asp 35 | Lys 66 | engineered mutation |
| Asp 112 | Lys 143 | engineered mutation |
| Asp 134 | Met 165 | engineered mutation |
| Cys 139 | Tyr 170 | engineered mutation |
| Met 157 | Ile 188 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 38533.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 723.5 | |
| All* | Total formula weight | 39256.5 |
