3QZU

Crystal structure of Bacillus subtilis Lipase A 7-fold mutant; the outcome of directed evolution towards thermostability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lipase estA	polymer	181	19266.5	2	UniProt (P37957) Pfam (PF01674) In PDB	Bacillus subtilis subsp. subtilis	Lipase A, Triacylglycerol lipase
2	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
5		water	water		18.0	482	Chemie (HOH)

Sequence modifications

A, B: 1 - 181 (UniProt: P37957)

PDB	External Database	Details
Gln 33	Arg 64	engineered mutation
Asn 34	Asp 65	engineered mutation
Asp 35	Lys 66	engineered mutation
Asp 112	Lys 143	engineered mutation
Asp 134	Met 165	engineered mutation
Cys 139	Tyr 170	engineered mutation
Met 157	Ile 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38533.0
Non-Polymers*	Number of molecules	9
	Total formula weight	723.5
All*	Total formula weight	39256.5

*Water molecules are not included.