3QMM

Structure of 6B, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lipase estA	polymer	181	19572.8	2	UniProt (P37957) Pfam (PF01674) In PDB	Bacillus subtilis	Lipase A, Triacylglycerol lipase
2		water	water		18.0	252	Chemie (HOH)

Sequence modifications

A, B: 1 - 181 (UniProt: P37957)

PDB	External Database	Details
Ser 15	Ala 46	engineered mutation
Ser 17	Phe 48	engineered mutation
Glu 20	Ala 51	engineered mutation
Tyr 89	Asn 120	engineered mutation
Asp 111	Gly 142	engineered mutation
Pro 114	Leu 145	engineered mutation
Asp 132	Ala 163	engineered mutation
Glu 134	Met 165	engineered mutation
Pro 137	Met 168	engineered mutation
Met 157	Ile 188	engineered mutation
Pro 163	Ser 194	engineered mutation
Tyr 166	Asn 197	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39145.7
All*	Total formula weight	39145.7

*Water molecules are not included.