3Q8M

Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in complex with substrate 5'-flap DNA and K+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Flap endonuclease 1	polymer	341	38467.2	2	UniProt (P39748) Pfam (PF00752) Pfam (PF00867)	Homo sapiens (human)	FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1
2	C, F (D, G)	DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3')	polymer	18	5476.6	2
3	D, G (E, H)	DNA (5'-D(*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3')	polymer	12	3742.5	2
4	E, H (F, I)	DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3')	polymer	7	2058.4	2
5	I, J (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
6	K, L, M, N, O... (A, B, D, E, F...)	water	water		18.0	110	Chemie (HOH)

Sequence modifications

A, B: 2 - 336 (UniProt: P39748)

PDB	External Database	Details
Ala 181	Asp 181	engineered mutation
Leu 337	-	expression tag
Glu 338	-	expression tag
Val 339	-	expression tag
Leu 340	-	expression tag
Phe 341	-	expression tag
Gln 342	-	expression tag

D, G: 1 - 17 (PDB: 3Q8M)

PDB	External Database	Details
Dt 18	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	99489.1
Non-Polymers*	Number of molecules	2
	Total formula weight	78.2
All*	Total formula weight	99567.3

*Water molecules are not included.