3Q8G
Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRAL-TRIO domain-containing protein YKL091C | polymer | 320 | 37355.7 | 1 | UniProt (P33324) Pfam (PF03765) Pfam (PF00650) | Saccharomyces cerevisiae (yeast) | |
| 2 | B (A) | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | non-polymer | 744.0 | 1 | Chemie (PEE) PubChem (108229) PubChem (6437392) PubChem (9546744) PubChem (44891344) PubChem (54095275) PubChem (14238554) PubChem (5909904) PubChem (9546757) PubChem (99655478) PubChem (99655479) PubChem (138113035) PubChem (171729549) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 4 | D (A) | water | water | 18.0 | 222 | Chemie (HOH) |
Sequence modifications
A: 1 - 310 (UniProt: P33324)
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | expression tag |
| Gly -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 126 | Glu 126 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 37355.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 836.1 | |
| All* | Total formula weight | 38191.9 |
