3Q8G

Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRAL-TRIO domain-containing protein YKL091C	polymer	320	37355.7	1	UniProt (P33324) Pfam (PF03765) Pfam (PF00650) In PDB	Saccharomyces cerevisiae (yeast)
2	A	1,2-dioleoyl-sn-glycero-3-phosphoethanolamine	non-polymer		744.0	1	Chemie (PEE)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	222	Chemie (HOH)

Sequence modifications

A: 1 - 310 (UniProt: P33324)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 126	Glu 126	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37355.7
Non-Polymers*	Number of molecules	2
	Total formula weight	836.1
All*	Total formula weight	38191.9

*Water molecules are not included.