3PZT

Structure of the endo-1,4-beta-glucanase from Bacillus subtilis 168 with manganese(II) ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endoglucanase	polymer	327	36148.2	2	UniProt (P10475) Pfam (PF00150) In PDB	Bacillus subtilis subsp. subtilis	Carboxymethyl-cellulase, CMCase, Cellulase, Endo-1,4-beta-glucanase
2	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5		water	water		18.0	452	Chemie (HOH)

Sequence modifications

A, B: 27 - 332 (UniProt: P10475)

PDB	External Database	Details
Met 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Val 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72296.4
Non-Polymers*	Number of molecules	6
	Total formula weight	481.1
All*	Total formula weight	72777.5

*Water molecules are not included.