3PZM

Structure of the hyperthermostable endo-1,4-beta-D-mannanase from Thermotoga petrophila RKU-1 with three glycerol molecules

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mannan endo-1,4-beta-mannosidase. Glycosyl Hydrolase family 5	polymer	383	44213.4	1	UniProt (A5IMX7) Pfam (PF00150)	Thermotoga petrophila RKU-1
2	B, C, E (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	D (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4	F (A)	water	water		18.0	363	Chemie (HOH)

Sequence modifications

A: 32 - 393 (UniProt: A5IMX7)

PDB	External Database	Details
Met 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Ser 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Leu 24	-	expression tag
Val 25	-	expression tag
Pro 26	-	expression tag
Arg 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag
His 30	-	expression tag
Met 31	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44213.4
Non-Polymers*	Number of molecules	4
	Total formula weight	398.4
All*	Total formula weight	44611.8

*Water molecules are not included.