3PX2

Structure of TylM1 from Streptomyces fradiae H123N mutant in complex with SAH and dTDP-Quip3N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	N-methyltransferase	polymer	262	28375.7	2	UniProt (P95748) Pfam (PF13649)	Streptomyces fradiae
2	C, E (A, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	D, F (A, D)	[(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate	non-polymer		547.3	2	Chemie (T3Q)
4	G, H (D)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	I, J (A, D)	water	water		18.0	414	Chemie (HOH)

Sequence modifications

A, D: 1 - 255 (UniProt: P95748)

PDB	External Database	Details
Asn 123	His 123	engineered mutation
Leu 256	-	expression tag
Glu 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56751.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1987.6
All*	Total formula weight	58739.0

*Water molecules are not included.