3PV0

Crystal Structure of a pre-translocation state MBP-Maltose transporter complex without nucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Maltose transporter subunit; periplasmic-binding component of ABC superfamily	polymer	370	40815.3	1	UniProt (B1XC33) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli
2	F	Maltose transporter subunit; membrane component of ABC superfamily	polymer	514	57052.9	1	UniProt (B1XC32) Pfam (PF20872) Pfam (PF14785) Pfam (PF00528) UniProt (by SIFTS) (P02916) In PDB	Escherichia coli
3	G	Maltose transporter subunit; membrane component of ABC superfamily	polymer	296	32246.2	1	UniProt (B1XC31) Pfam (PF00528) UniProt (by SIFTS) (P68183) In PDB	Escherichia coli
4	A, B	Fused maltose transport subunit, ATP-binding component of ABC superfamily; regulatory protein	polymer	381	42184.5	2	UniProt (B1XC34) Pfam (PF00005) Pfam (PF08402) UniProt (by SIFTS) (P68187) In PDB	Escherichia coli
5	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
6	F	(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE	non-polymer		749.0	1	Chemie (PGV)

Sequence modifications

E: 1 - 370 (UniProt: B1XC33)

PDB	External Database	Details
Cys 69	Gly 95	engineered mutation
Cys 337	Ser 363	engineered mutation

A, B: 1 - 371 (UniProt: B1XC34)

PDB	External Database	Details
Ala 372	-	expression tag
Ser 373	-	expression tag
Ala 374	-	expression tag
Ser 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	214483.5
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	1
	Total formula weight	749.0
All*	Total formula weight	215917.1

*Water molecules are not included.