3PPC

Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, tyrosine variant with zinc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase	polymer	789	88465.3	2	UniProt (P82610) Pfam (PF08267) Pfam (PF01717)	Candida albicans (Yeast)	Cobalamin-independent methionine synthase, Methionine synthase, vitamin-B12 independent isozyme
2	C, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D, E, F, G, H... (A, B)	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
4	M, N (A, B)	water	water		18.0	433	Chemie (HOH)

Sequence modifications

A, B: 1 - 767 (UniProt: P82610)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Tyr 103	Lys 103	engineered mutation
Tyr 104	Lys 104	engineered mutation
Tyr 107	Glu 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	176930.5
Non-Polymers*	Number of molecules	10
	Total formula weight	414.4
All*	Total formula weight	177344.9

*Water molecules are not included.