3POC
The crystal structure of the D307A mutant of alpha-Glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with acarbose
Replaces: 3NQQEntity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | alpha-Glucosidase | polymer | 666 | 77405.5 | 2 | UniProt (A5ZY13) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) | Ruminococcus obeum | |
| 2 | C (C) | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 645.6 | 1 | In PDB BIRD (PRD_900007) GlyTouCan (G66431MI) | alpha-acarbose | ||
| 3 | D (A) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 4 | E, G (A, B) | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose | non-polymer | 321.3 | 2 | Chemie (AC1) | |||
| 5 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | H, I (A, B) | water | water | 18.0 | 516 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 663 (UniProt: A5ZY13)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 307 | Asp 307 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 154810.9 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 645.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 914.9 | |
| All* | Total formula weight | 156371.4 |
