3PO3
Arrested RNA Polymerase II reactivation intermediate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase II subunit RPB1 | polymer | 1733 | 191821.6 | 1 | UniProt (P04050) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit 1, RNA polymerase II subunit B1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220 |
| 2 | B (B) | DNA-directed RNA polymerase II subunit RPB2 | polymer | 1224 | 138937.3 | 1 | UniProt (P08518) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit 2, B150, DNA-directed RNA polymerase II 140 kDa polypeptide |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 318 | 35330.5 | 1 | UniProt (P16370) Pfam (PF01193) Pfam (PF01000) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit 3, RNA polymerase II subunit B3, B44.5, DNA-directed RNA polymerase II 45 kDa polypeptide |
| 4 | D (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 221 | 25451.2 | 1 | UniProt (P20433) Pfam (PF03874) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit B4, B32, DNA-directed RNA polymerase II 32 kDa polypeptide |
| 5 | E (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 215 | 25117.1 | 1 | UniProt (P20434) Pfam (PF03871) Pfam (PF01191) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerases I, II, and III subunit ABC1, ABC27, DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 155 | 17931.8 | 1 | UniProt (P20435) Pfam (PF01192) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerases I, II, and III subunit ABC2, ABC23, DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide |
| 7 | G (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 171 | 19081.1 | 1 | UniProt (P34087) Pfam (PF03876) Pfam (PF00575) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit B7, B16 |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 146 | 16525.4 | 1 | UniProt (P20436) Pfam (PF03870) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerases I, II, and III subunit ABC3, ABC14.4, ABC14.5, DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 122 | 14308.2 | 1 | UniProt (P27999) Pfam (PF02150) Pfam (PF01096) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit B9, B12.6, DNA-directed RNA polymerase II 14.2 kDa polypeptide, DNA-directed RNA polymerase II subunit 9 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 70 | 8290.7 | 1 | UniProt (P22139) Pfam (PF01194) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerases I, II, and III subunit ABC5, ABC10-beta, ABC8, DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11 | polymer | 120 | 13633.5 | 1 | UniProt (P38902) Pfam (PF13656) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerase II subunit B11, B13.6, DNA-directed RNA polymerase II 13.6 kDa polypeptide |
| 12 | L (L) | DNA-directed RNA polymerases I, II, and III subunit RPABC4 | polymer | 70 | 7730.0 | 1 | UniProt (P40422) Pfam (PF03604) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | RNA polymerases I, II, and III subunit ABC4, ABC10-alpha |
| 13 | M (N) | DNA non-template strand | polymer | 14 | 4344.8 | 1 | |||
| 14 | N (P) | RNA product strand | polymer | 5 | 1481.0 | 1 | |||
| 15 | O (S) | Transcription elongation factor S-II | polymer | 178 | 20072.8 | 1 | UniProt (P07273) Pfam (PF07500) Pfam (PF01096) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | DNA strand transfer protein alpha, STP-alpha, DNA strand transferase 1, Pyrimidine pathway regulatory protein 2 |
| 16 | P (T) | DNA template strand | polymer | 27 | 8323.2 | 1 | |||
| 17 | AA, BA, DA, Q, R... (J, L, S, A, B...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 18 | S (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 19 | T, W (A, B) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 20 | U (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 21 | CA (L) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) |
Sequence modifications
S: 132 - 309 (UniProt: P07273)
| PDB | External Database | Details |
|---|---|---|
| Ala 290 | Asp 290 | engineered mutation |
| Ala 291 | Glu 291 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 548380.0 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1119.6 | |
| All* | Total formula weight | 549499.5 |
