3PJQ
Trypanosoma cruzi trans-sialidase-like inactive isoform (including the natural mutation Tyr342His) in complex with lactose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Trans-sialidase | polymer | 648 | 71364.2 | 1 | UniProt (Q26966) Pfam (PF13859) Pfam (PF22925) In PDB | Trypanosoma cruzi | |
2 | B | beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900008) GlyTouCan (G88362QR) | alpha-lactose | ||
3 | water | water | 18.0 | 213 | Chemie (HOH) |
Sequence modifications
A: 1 - 634 (UniProt: Q26966)
PDB | External Database | Details |
---|---|---|
Met -13 | - | initiating methionine |
Gly -12 | - | expression tag |
Gly -11 | - | expression tag |
Ser -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Met -2 | - | expression tag |
Ala -1 | - | expression tag |
Ser 0 | - | expression tag |
Phe 58 | Asn 59 | engineered mutation |
Thr 262 | Ser 263 | SEE REMARK 999 |
His 342 | Tyr 343 | SEE REMARK 999 |
His 476 | Arg 477 | SEE REMARK 999 |
Leu 484 | Val 485 | SEE REMARK 999 |
Lys 495 | Ser 496 | engineered mutation |
Gly 496 | Val 497 | engineered mutation |
Lys 520 | Glu 521 | engineered mutation |
Val 558 | Glu 559 | SEE REMARK 999 |
Gly 593 | Asp 594 | engineered mutation |
Asp 597 | Ile 598 | engineered mutation |
Arg 599 | His 600 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 71364.2 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
All* | Total formula weight | 71706.5 |