3OYI

Crystal structure of the PFV S217Q mutant intasome in complex with manganese

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PFV integrase	polymer	395	44497.7	2	UniProt (P14350) Pfam (PF17921) Pfam (PF00665) Pfam (PF18103)	Human spumaretrovirus (SFVcpz(hu))	p42In
2	C (C)	DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')	polymer	19	5834.8	1
3	D (D)	DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*A)-3')	polymer	17	5195.4	1
4	E (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	F, M (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
6	G, N (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	H, I, J, K (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	L (A)	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
9	O, P, Q, R (A, B, C, D)	water	water		18.0	205	Chemie (HOH)

Sequence modifications

A, B: 1 - 392 (UniProt: P14350)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Gln 217	Gly 968	engineered mutation
Gly 218	Ser 969	variant

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100025.7
Non-Polymers*	Number of molecules	10
	Total formula weight	753.8
All*	Total formula weight	100779.5

*Water molecules are not included.