3OWV
Structural insights into catalytic and substrate binding mechanisms of the strategic EndA nuclease from Streptococcus pneumoniae
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-entry nuclease | polymer | 248 | 26779.7 | 2 | UniProt (P0A3S3) Pfam (PF01223) | Streptococcus pneumoniae | Competence-specific nuclease |
| 2 | C, F (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D, E, G, H, I (A, B) | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
| 4 | J, K (A, B) | water | water | 18.0 | 495 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 274 (UniProt: P0A3S3)
| PDB | External Database | Details |
|---|---|---|
| Gly 27 | - | expression tag |
| Ala 28 | - | expression tag |
| Met 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Ala 160 | His 160 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 53559.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 225.9 | |
| All* | Total formula weight | 53785.3 |
