3OSQ

Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 175

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein,Green fluorescent protein	polymer	663	73705.0	1	UniProt (P0AEY0) UniProt (P42212) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C, D (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E (A)	water	water		18.0	294	Chemie (HOH)

Sequence modifications

A: 1 - 173 (UniProt: P0AEY0)

PDB	External Database	Details
Met -35	-	expression tag
Arg -34	-	expression tag
Gly -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Gly -25	-	expression tag
Met -24	-	expression tag
Ala -23	-	expression tag
Ser -22	-	expression tag
Met -21	-	expression tag
Thr -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gln -17	-	expression tag
Gln -16	-	expression tag
Met -15	-	expression tag
Gly -14	-	expression tag
Arg -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Arg -3	-	expression tag
Trp -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

A: 176 - 267 (UniProt: P42212)

PDB	External Database	Details
Ala 192	Val 163	conflict
Gly 204	Ser 175	conflict
Tyr 209	Asp 180	conflict
Lys 235	Ala 206	conflict
Leu 260	His 231	conflict

A: 276 - 420 (UniProt: P42212)

PDB	External Database	Details
Gly 268	-	linker
Gly 269	-	linker
Thr 270	-	linker
Gly 271	-	linker
Gly 272	-	linker
Ser 273	-	linker
Met 274	-	linker
Val 275	-	linker
Leu 338	Phe 64	conflict
C12 340	Ser 65	chromophore
C12 340	Tyr 66	chromophore
C12 340	Gly 67	chromophore
Ile 367	Val 93	conflict
Phe 419	Tyr 145	conflict
Gly 421	-	linker

A: 422 - 618 (UniProt: P0AEY0)

PDB	External Database	Details
His 174	-	linker
Leu 175	-	linker
Val 560	Ala 338	conflict
Asp 608	Ala 386	conflict
Gly 619	-	expression tag
Ser 620	-	expression tag
His 621	-	expression tag
His 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag
His 625	-	expression tag
His 626	-	expression tag
Gly 627	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	73705.0
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	74239.4

*Water molecules are not included.