3OO7

Crystal structures and biochemical characterization of the bacterial solute receptor AcbH reveal an unprecedented exclusive substrate preference for b-D-galactopyranose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ABC transporter binding protein AcbH	polymer	415	45879.4	1	UniProt (Q27GR2) Pfam (PF01547)	Actinoplanes
2	B, C, D, E (A)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F, G, H, I, J... (A)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	L (A)	water	water		18.0	308	Chemie (HOH)

Sequence modifications

A: 10 - 404 (UniProt: Q27GR2)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
Gly 2	-	expression tag
Leu 3	-	expression tag
Val 4	-	expression tag
Pro 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
His 9	-	expression tag
Met 10	Thr 39	engineered mutation
Lys 361	Asp 390	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45879.4
Non-Polymers*	Number of molecules	10
	Total formula weight	936.8
All*	Total formula weight	46816.3

*Water molecules are not included.