3OL2

Receptor-ligand structure of Human Semaphorin 4D with Plexin B1.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Semaphorin-4D	polymer	663	74315.5	1	UniProt (Q92854) Pfam (PF01403) Pfam (PF01437) Pfam (PF00047)	Homo sapiens (human)	BB18, A8, GR3
2	B (B)	Plexin-B1	polymer	528	56924.7	1	UniProt (O43157) Pfam (PF01403) Pfam (PF01437)	Homo sapiens (human)	Semaphorin receptor SEP
3	C, E, H (C, E, H)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
4	D, F, G (D, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	I (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 22 - 677 (UniProt: Q92854)

PDB	External Database	Details
Lys 678	-	expression tag
His 679	-	expression tag
His 680	-	expression tag
His 681	-	expression tag
His 682	-	expression tag
His 683	-	expression tag
His 684	-	expression tag

B: 20 - 535 (UniProt: O43157)

PDB	External Database	Details
Glu 17	-	cloning artifact
Thr 18	-	cloning artifact
Gly 19	-	cloning artifact
Gly 536	-	expression tag
Thr 537	-	expression tag
Lys 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	131240.2
Branched	Number of molecules	6
	Total formula weight	3032.8
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	134494.3

*Water molecules are not included.