3OIN

Crystal structure of Saccharomyces cerevisiae Nep1/Emg1 bound to S-adenosylhomocysteine and 1 molecule of cognate RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribosomal RNA small subunit methyltransferase NEP1	polymer	253	28035.5	2	UniProt (Q06287) Pfam (PF03587)	Saccharomyces cerevisiae (Baker's yeast)	18S rRNA (pseudouridine(1189)-N1)-methyltransferase,18S rRNA Psi1189 methyltransferase,Essential for mitotic growth protein 1,Nucleolar essential protein 1
2	C (C)	5'-R(*GP*GP*GP*CP*UP*UP*CP*AP*AP*CP*GP*CP*CP*C)-3'	polymer	14	4437.7	1		Saccharomyces cerevisiae
3	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
4	F (B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (25210495) PubChem (13792) PubChem (177678424) PubChem (44361184) PubChem (21125603) PubChem (46936206) PubChem (50989525) PubChem (25203782) PubChem (51380914) PubChem (52224398) PubChem (189149) PubChem (57544532) PubChem (439155) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (52224400) PubChem (5284331) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (71170554) PubChem (13231890) PubChem (164157219) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
5	G (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
6	H (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
7	I, J, K (A, B, C)	water	water		18.0	241	Chemie (HOH)

Sequence modifications

A, B: 1 - 252 (UniProt: Q06287)

PDB	External Database	Details
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	60508.8
Non-Polymers*	Number of molecules	5
	Total formula weight	628.4
All*	Total formula weight	61137.2

*Water molecules are not included.