3OG7

B-Raf Kinase V600E oncogenic mutant in complex with PLX4032

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	AKAP9-BRAF fusion protein	polymer	289	32977.7	2	UniProt (Q5IBP5) Pfam (PF07714) UniProt (by SIFTS) (P15056) In PDB	Homo sapiens (human)	Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
2	A	N-(3-{[5-(4-chlorophenyl)-1H-pyrrolo[2,3-b]pyridin-3-yl]carbonyl}-2,4-difluorophenyl)propane-1-sulfonamide	non-polymer		489.9	1	Chemie (032)
3		water	water		18.0	65	Chemie (HOH)

Sequence modifications

A, B: 449 - 720 (UniProt: Q5IBP5)

PDB	External Database	Details
Met 432	-	expression tag
Lys 433	-	expression tag
Lys 434	-	expression tag
Gly 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
Gly 442	-	expression tag
Ser 443	-	expression tag
Arg 444	-	expression tag
Asp 445	-	expression tag
Ala 446	-	expression tag
Ala 447	-	expression tag
Asp 448	-	expression tag
Ala 522	Lys 1248	engineered mutation
Ala 543	Ile 1269	engineered mutation
Ser 544	Ile 1270	engineered mutation
Lys 551	Ile 1277	engineered mutation
Arg 562	Gln 1288	engineered mutation
Asn 588	Leu 1314	engineered mutation
Glu 600	Val 1326	variant
Ser 630	Lys 1356	engineered mutation
Glu 667	Phe 1393	engineered mutation
Ser 673	Tyr 1399	engineered mutation
Arg 688	Ala 1414	engineered mutation
Ser 706	Leu 1432	engineered mutation
Arg 709	Gln 1435	engineered mutation
Glu 713	Ser 1439	engineered mutation
Glu 716	Leu 1442	engineered mutation
Glu 720	Ser 1446	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65955.5
Non-Polymers*	Number of molecules	1
	Total formula weight	489.9
All*	Total formula weight	66445.4

*Water molecules are not included.