3OEB
Crystal structure of the Q121E mutant of C.polysaccharolyticus CBM16-1 bound to mannopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | S-layer associated multidomain endoglucanase | polymer | 144 | 15825.6 | 1 | UniProt (Q9ZA17) Pfam (PF02018) In PDB | Caldanaerobius polysaccharolyticus | |
| 2 | B | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-alpha-D-mannopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G58879KH) | |||
| 3 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | water | water | 18.0 | 142 | Chemie (HOH) |
Sequence modifications
A: 2 - 144 (UniProt: Q9ZA17)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Glu 121 | Gln 733 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 15825.6 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 828.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 232.2 | |
| All* | Total formula weight | 16886.5 |
