3OEA
Crystal structure of the Q121E mutants of C.polysaccharolyticus CBM16-1 bound to cellopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | S-layer associated multidomain endoglucanase | polymer | 146 | 16050.8 | 2 | UniProt (Q9ZA17) Pfam (PF02018) | Caldanaerobius polysaccharolyticus | |
| 2 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 2 | In PDB GlyTouCan (G75287MT) | |||
| 3 | E, F (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 497 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 144 (UniProt: Q9ZA17)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Met 1 | - | expression tag |
| Glu 121 | Gln 733 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 32101.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1657.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 33839.2 |
