3OE7

Structure of four mutant forms of yeast f1 ATPase: gamma-I270T

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, J, K... (A, B, C, J, K...)	ATP synthase subunit alpha	polymer	510	55007.4	9	UniProt (P07251) Pfam (PF02874) Pfam (PF00006) Pfam (PF00306)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)
2	D, E, F, M, N... (D, E, F, M, N...)	ATP synthase subunit beta	polymer	484	52010.0	9	UniProt (P00830) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)
3	G, P, Y (G, P, Y)	ATP synthase subunit gamma	polymer	278	30645.1	3	UniProt (P38077) Pfam (PF00231)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	F-ATPase gamma subunit
4	H, Q, Z (H, Q, Z)	ATP synthase subunit delta	polymer	137	14494.3	3	UniProt (Q12165) Pfam (PF21334) Pfam (PF02823)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	F-ATPase delta subunit
5	AA, I, R (I, R, 1)	ATP synthase subunit epsilon	polymer	61	6618.4	3	UniProt (P21306) Pfam (PF04627)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	ATPase subunit epsilon
6	AB, BA, CB, DA, EB... (U, A, V, B, X...)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	15	Chemie (ANP)
7	BB, CA, DB, EA, FB... (U, A, V, B, X...)	MAGNESIUM ION	non-polymer		24.3	15	Chemie (MG)
8	TA (N)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)

Sequence modifications

D, E, F, M, N, O, V, W, X: 3 - 478 (UniProt: P00830)

PDB	External Database	Details
Ala -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag

G, P, Y: 1 - 278 (UniProt: P38077)

PDB	External Database	Details
Thr 270	Ile 303	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	27
	Total formula weight	1118429.6
Non-Polymers*	Number of molecules	31
	Total formula weight	8052.5
All*	Total formula weight	1126482.1

*Water molecules are not included.