3OAB

Mint deletion mutant of heterotetrameric geranyl pyrophosphate synthase in complex with ligands

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Geranyl diphosphate synthase large subunit	polymer	295	31947.0	2	UniProt (Q9SBR3) Pfam (PF00348)	Mentha x piperita (Peppermint)	Geranyl pyrophosphate synthase large subunit, Gpp synthase large subunit
2	B, C (B, C)	Geranyl diphosphate synthase small subunit	polymer	264	28712.8	2	UniProt (Q9SBR4)	Mentha x piperita (Peppermint)	Gpp synthase small subunit
3	E (A)	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
4	F, K (A, D)	DIMETHYLALLYL S-THIOLODIPHOSPHATE	non-polymer		262.2	2	Chemie (DST)
5	G, H, L, M (A, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	I (C)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	J (D)	3-METHYLBUT-3-ENYL TRIHYDROGEN DIPHOSPHATE	non-polymer		246.1	1	Chemie (IPE)
8	N, O, P, Q (A, B, C, D)	water	water		18.0	636	Chemie (HOH)

Sequence modifications

A, D: 2 - 295 (UniProt: Q9SBR3)

PDB	External Database	Details
Met 1	-	expression tag

B, C: 2 - 266 (UniProt: Q9SBR4)

PDB	External Database	Details
Met 1	-	expression tag
-	Leu 131	deletion
-	Thr 132	deletion
-	Asp 133	deletion
-	Gly 134	deletion
-	Ser 135	deletion
-	Arg 136	deletion
-	Pro 137	deletion
-	Val 138	deletion
-	Ser 139	deletion
-	Lys 140	deletion
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	121319.6
Non-Polymers*	Number of molecules	9
	Total formula weight	1107.7
All*	Total formula weight	122427.3

*Water molecules are not included.