3O3Q

Crystal structure of "L44F/M67I/L73V/A103G/deletion 104-106/F108Y/V109L/L111I/C117V/R119G/deletion 120-122" mutant form of Human acidic fibroblast growth factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Heparin-binding growth factor 1	polymer	140	15916.8	4	UniProt (P05230) Pfam (PF00167)	Homo sapiens (human)	HBGF-1, Acidic fibroblast growth factor, aFGF, Beta-endothelial cell growth factor, ECGF-beta
2	E, F (B, D)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	G, H, I, J (A, B, C, D)	water	water		18.0	578	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 140 (UniProt: P05230)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 44	Leu 59	engineered mutation
Ile 67	Met 82	engineered mutation
Val 73	Leu 88	engineered mutation
Gly 103	Ala 118	engineered mutation
-	Glu 119	deletion
-	Lys 120	deletion
-	Asn 121	deletion
Tyr 108	Phe 123	engineered mutation
Leu 109	Val 124	engineered mutation
Ile 111	Leu 126	engineered mutation
Val 117	Cys 132	engineered mutation
Gly 119	Arg 134	engineered mutation
-	Gly 135	deletion
-	Pro 136	deletion
-	Arg 137	deletion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	63667.2
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	63851.3

*Water molecules are not included.