3O3L

Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol (1,3,4,5)tetrakisphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Myo-inositol hexaphosphate phosphohydrolase	polymer	340	38971.8	2	UniProt (Q7WUJ1)	Selenomonas ruminantium	PTP-like phytase
2	C, D, E, L, M (A, B)	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
3	F, G, H, I, N... (A, B)	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
4	J, R (A, B)	INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE	non-polymer		500.1	2	Chemie (4IP)
5	K (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	S, T (A, B)	water	water		18.0	766	Chemie (HOH)

Sequence modifications

A, B: 28 - 346 (UniProt: Q7WUJ1)

PDB	External Database	Details
Met 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Arg 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag
Ser 252	Cys 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77943.7
Non-Polymers*	Number of molecules	16
	Total formula weight	2067.6
All*	Total formula weight	80011.3

*Water molecules are not included.