3O2Q

Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Symplekin	polymer	351	39647.0	2	UniProt (Q92797) Pfam (PF11935) In PDB	Homo sapiens (human)
2	B, E	RNA polymerase II subunit A C-terminal domain phosphatase SSU72	polymer	214	24758.8	2	UniProt (Q9NP77) Pfam (PF04722) In PDB	Homo sapiens (human)	CTD phosphatase SSU72
3	F	RNA polymerase II CTD Serine-5 phosphopeptide	polymer	6	730.7	1
4	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5		water	water		18.0	250	Chemie (HOH)

Sequence modifications

A, D: 30 - 360 (UniProt: Q92797)

PDB	External Database	Details
Met 10	-	EXPRESSION TAG
Gly 11	-	EXPRESSION TAG
Ser 12	-	EXPRESSION TAG
Ser 13	-	EXPRESSION TAG
His 14	-	EXPRESSION TAG
His 15	-	EXPRESSION TAG
His 16	-	EXPRESSION TAG
His 17	-	EXPRESSION TAG
His 18	-	EXPRESSION TAG
His 19	-	EXPRESSION TAG
Ser 20	-	EXPRESSION TAG
Ser 21	-	EXPRESSION TAG
Gly 22	-	EXPRESSION TAG
Leu 23	-	EXPRESSION TAG
Val 24	-	EXPRESSION TAG
Pro 25	-	EXPRESSION TAG
Arg 26	-	EXPRESSION TAG
Gly 27	-	EXPRESSION TAG
Ser 28	-	EXPRESSION TAG
His 29	-	EXPRESSION TAG

B, E: 1 - 194 (UniProt: Q9NP77)

PDB	External Database	Details
Met -19	-	EXPRESSION TAG
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
His -10	-	EXPRESSION TAG
Ser -9	-	EXPRESSION TAG
Ser -8	-	EXPRESSION TAG
Gly -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Val -5	-	EXPRESSION TAG
Pro -4	-	EXPRESSION TAG
Arg -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Ser -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
Ser 12	Cys 12	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	129542.1
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	129637.1

*Water molecules are not included.