3NV0

Crystal structure and mutational analysis of the NXF2/NXT1 heterodimeric complex from caenorhabditis elegans at 1.84 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nuclear RNA export factor 2	polymer	205	23529.5	1	UniProt (Q9XVS8) Pfam (PF22602)	Caenorhabditis elegans (nematode)
2	B (B)	NTF2-related export protein	polymer	154	17360.3	1	UniProt (Q9U757) Pfam (PF02136)	Caenorhabditis elegans (nematode)
3	C, D, R, S, T (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	5	Chemie (PEG)
4	E (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	AA, BA, F, G, H... (B, A)	SODIUM ION	non-polymer		23.0	19	Chemie (NA)
6	Q (B)	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
7	CA, DA (A, B)	water	water		18.0	292	Chemie (HOH)

Sequence modifications

A: 202 - 405 (UniProt: Q9XVS8)

PDB	External Database	Details
Met 201	-	expression tag

B: 2 - 137 (UniProt: Q9U757)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Ser -6	-	expression tag
Gln -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Asn -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40889.8
Non-Polymers*	Number of molecules	26
	Total formula weight	1107.6
All*	Total formula weight	41997.4

*Water molecules are not included.