3NT7

Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, A)	Uracil-DNA glycosylase	polymer	238	27196.0	2	UniProt (Q91UM2) UniProt (by SIFTS) (P04303)	VACCINIA VIRUS WESTERN RESERVE (VACV)	UDG
2	C, D (C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	E, F (C, A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H (C, A)	water	water		18.0	73	Chemie (HOH)

Sequence modifications

C, A: 1 - 218 (UniProt: Q91UM2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Val 187	Arg 187	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54392.1
Non-Polymers*	Number of molecules	4
	Total formula weight	376.3
All*	Total formula weight	54768.4

*Water molecules are not included.