3NK3

Crystal structure of full-length sperm receptor ZP3 at 2.6 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Zona pellucida 3	polymer	297	32673.7	2	UniProt (P79762) Pfam (PF00100)	Gallus gallus (bantam chickens)
2	C, D (C, D)	Zona pellucida 3	polymer	30	3129.5	2	UniProt (P79762)	Gallus gallus (bantam chickens)
3	E (E)	beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose	branched		383.3	1	In PDB BIRD (PRD_900084) GlyTouCan (G00031MO)		Thomsen-Friedenreich antigen
4	F (C)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
5	G, H, I, J (A, B, C, D)	water	water		18.0	127	Chemie (HOH)

Sequence modifications

A, B: 51 - 347 (UniProt: P79762)

PDB	External Database	Details
-	Pro 23	deletion
-	Trp 24	deletion
-	Asp 25	deletion
-	Ile 26	deletion
-	Ser 27	deletion
-	Trp 28	deletion
-	Ala 29	deletion
-	Ala 30	deletion
-	Arg 31	deletion
-	Gly 32	deletion
-	Asp 33	deletion
-	Pro 34	deletion
-	Ser 35	deletion
-	Ala 36	deletion
-	Trp 37	deletion
-	Ser 38	deletion
-	Trp 39	deletion
-	Gly 40	deletion
-	Ala 41	deletion
-	Glu 42	deletion
-	Ala 43	deletion
-	His 44	deletion
-	Ser 45	deletion
-	Arg 46	deletion
-	Ala 47	deletion
-	Val 48	deletion
-	Ala 49	deletion
-	Gly 50	deletion
-	Ser 51	deletion
-	His 52	deletion
Gln 159	Asn 159	engineered mutation

C, D: 359 - 382 (UniProt: P79762)

PDB	External Database	Details
Ala 359	Arg 359	engineered mutation
Ala 361	Arg 361	engineered mutation
Ala 362	Arg 362	engineered mutation
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	71606.3
Branched	Number of molecules	1
	Total formula weight	383.3
Non-Polymers*	Number of molecules	1
	Total formula weight	189.1
All*	Total formula weight	72178.8

*Water molecules are not included.