3NH5
Crystal structure of E177A-mutant murine aminoacylase 3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Aspartoacylase-2 | polymer | 327 | 36435.4 | 1 | UniProt (Q91XE4) Pfam (PF04952) | Mus musculus (mouse) | Aminoacylase-3, ACY-3, Aminoacylase III, Acylase III, Hepatitis C virus core-binding protein 1, HCBP1 |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 4 | E (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 5 | F (A) | FORMIC ACID | non-polymer | 46.0 | 1 | Chemie (FMT) | |||
| 6 | G (A) | water | water | 18.0 | 70 | Chemie (HOH) |
Sequence modifications
A: 1 - 318 (UniProt: Q91XE4)
| PDB | External Database | Details |
|---|---|---|
| Met -8 | - | expression tag |
| Trp -7 | - | expression tag |
| Ser -6 | - | expression tag |
| His -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Ala 177 | Glu 177 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 36435.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 241.4 | |
| All* | Total formula weight | 36676.8 |
