3NBZ

Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Exportin-1	polymer	1073	123367.2	2	UniProt (Q6P5F9) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767)	Mus musculus (mouse)	Exp1, Chromosome region maintenance 1 protein homolog
2	B, E (B, E)	Snurportin-1	polymer	362	41431.9	2	UniProt (O95149) Pfam (PF11538) Pfam (PF21974)	Homo sapiens (human)	RNA U transporter 1
3	C, F (C, F)	GTP-binding nuclear protein Ran	polymer	176	20192.5	2	UniProt (P62826) Pfam (PF00071)	Homo sapiens (human)	GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24
4	G, L (C, F)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	H, K (C, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	I, J (C, D)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	785	Chemie (HOH)

Sequence modifications

A, D: 1 - 1071 (UniProt: Q6P5F9)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

B, E: 15 - 360 (UniProt: O95149)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 1	-	expression tag
Val 2	-	expression tag
Pro 3	-	expression tag
Leu 4	-	expression tag
Gln 5	-	expression tag
Leu 6	-	expression tag
Pro 7	-	expression tag
Pro 8	-	expression tag
Leu 9	-	expression tag
Glu 10	-	expression tag
Arg 11	-	expression tag
Leu 12	-	expression tag
Thr 13	-	expression tag
Leu 14	-	expression tag

C, F: 5 - 180 (UniProt: P62826)

PDB	External Database	Details
Leu 69	Gln 69	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	369983.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1140.9
All*	Total formula weight	371124.2

*Water molecules are not included.