3N97

RNA polymerase alpha C-terminal domain (E. coli) and sigma region 4 (T. aq. mutant) bound to (UP,-35 element) DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	RNA polymerase sigma factor	polymer	72	8527.9	2	UniProt (Q9EZJ8) Pfam (PF04545)	Thermus aquaticus
2	C, D (B, C)	DNA-directed RNA polymerase subunit alpha	polymer	84	9364.8	2	UniProt (P0A7Z4) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha, Transcriptase subunit alpha, RNA polymerase subunit alpha
3	E (M)	DNA (5'-D(*TP*GP*GP*AP*AP*AP*AP*AP*AP*GP*TP*AP*CP*TP*TP*GP*AP*CP*AP*TP*GP*G)-3')	polymer	22	6848.5	1
4	F (N)	DNA (5'-D(*CP*CP*AP*TP*GP*TP*CP*AP*AP*GP*TP*AP*CP*TP*TP*TP*TP*TP*TP*CP*C)-3')	polymer	21	6339.1	1
5	G (C)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	H (C)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, D: 366 - 437 (UniProt: Q9EZJ8)

PDB	External Database	Details
Met 386	Leu 386	engineered mutation
-	Lys 424	SEE REMARK 999
Arg 424	Tyr 425	SEE REMARK 999
Pro 426	Glu 427	SEE REMARK 999
Ser 429	Thr 430	engineered mutation
Glu 430	Arg 431	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	48972.9
Non-Polymers*	Number of molecules	1
	Total formula weight	59.0
All*	Total formula weight	49031.9

*Water molecules are not included.