3N97
RNA polymerase alpha C-terminal domain (E. coli) and sigma region 4 (T. aq. mutant) bound to (UP,-35 element) DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | RNA polymerase sigma factor | polymer | 72 | 8527.9 | 2 | UniProt (Q9EZJ8) Pfam (PF04545) In PDB | Thermus aquaticus | |
2 | B, C | DNA-directed RNA polymerase subunit alpha | polymer | 84 | 9364.8 | 2 | UniProt (P0A7Z4) Pfam (PF03118) In PDB | Escherichia coli | RNAP subunit alpha, Transcriptase subunit alpha, RNA polymerase subunit alpha |
3 | M | DNA (5'-D(*TP*GP*GP*AP*AP*AP*AP*AP*AP*GP*TP*AP*CP*TP*TP*GP*AP*CP*AP*TP*GP*G)-3') | polymer | 22 | 6848.5 | 1 | |||
4 | N | DNA (5'-D(*CP*CP*AP*TP*GP*TP*CP*AP*AP*GP*TP*AP*CP*TP*TP*TP*TP*TP*TP*CP*C)-3') | polymer | 21 | 6339.1 | 1 | |||
5 | C | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
6 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A, D: 366 - 437 (UniProt: Q9EZJ8)
PDB | External Database | Details |
---|---|---|
Met 386 | Leu 386 | engineered mutation |
- | Lys 424 | SEE REMARK 999 |
Arg 424 | Tyr 425 | SEE REMARK 999 |
Pro 426 | Glu 427 | SEE REMARK 999 |
Ser 429 | Thr 430 | engineered mutation |
Glu 430 | Arg 431 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 48972.9 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 59.0 | |
All* | Total formula weight | 49031.9 |