3N57
Crystal Structure of human Insulin-degrading enzyme (IDE) in complex with human atrial natriuretic peptide (ANP)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Insulin-degrading enzyme | polymer | 990 | 114560.6 | 2 | UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) In PDB | Homo sapiens (human) | Insulin protease, Insulinase, Insulysin, Abeta-degrading protease |
2 | C, D | Atrial natriuretic factor | polymer | 28 | 3087.5 | 2 | UniProt (P01160) Pfam (PF00212) In PDB | Homo sapiens (human) | ANF, Atrial natriuretic peptide, ANP, Prepronatriodilatin, CDD-ANF, Cardiodilatin-related peptide, CDP |
3 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
4 | water | water | 18.0 | 64 | Chemie (HOH) |
Sequence modifications
A, B: 42 - 1019 (UniProt: P14735)
PDB | External Database | Details |
---|---|---|
Met 30 | - | expression tag |
His 31 | - | expression tag |
His 32 | - | expression tag |
His 33 | - | expression tag |
His 34 | - | expression tag |
His 35 | - | expression tag |
His 36 | - | expression tag |
Ala 37 | - | expression tag |
Ala 38 | - | expression tag |
Gly 39 | - | expression tag |
Ile 40 | - | expression tag |
Pro 41 | - | expression tag |
Leu 110 | Cys 110 | engineered mutation |
Gln 111 | Glu 111 | engineered mutation |
Ser 171 | Cys 171 | engineered mutation |
Ala 178 | Cys 178 | engineered mutation |
Val 257 | Cys 257 | engineered mutation |
Leu 414 | Cys 414 | engineered mutation |
Asn 573 | Cys 573 | engineered mutation |
Ser 590 | Cys 590 | engineered mutation |
Ser 789 | Cys 789 | engineered mutation |
Ala 812 | Cys 812 | engineered mutation |
Ala 819 | Cys 819 | engineered mutation |
Ser 904 | Cys 904 | engineered mutation |
Asn 966 | Cys 966 | engineered mutation |
Ala 974 | Cys 974 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 235296.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 130.8 | |
All* | Total formula weight | 235427.0 |