3N0G
Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens) in complex with three Mg2+ ions and dimethylallyl-S-thiolodiphosphate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Isoprene synthase | polymer | 555 | 64118.0 | 2 | UniProt (Q9AR86) Pfam (PF01397) Pfam (PF03936) In PDB | Populus tremula x Populus alba (gray poplar) | |
2 | A, B | DIMETHYLALLYL S-THIOLODIPHOSPHATE | non-polymer | 262.2 | 2 | Chemie (DST) | |||
3 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
4 | water | water | 18.0 | 322 | Chemie (HOH) |
Sequence modifications
A, B: 53 - 595 (UniProt: Q9AR86)
PDB | External Database | Details |
---|---|---|
Met 41 | - | expression tag |
Arg 42 | - | expression tag |
Gly 43 | - | expression tag |
Ser 44 | - | expression tag |
His 45 | - | expression tag |
His 46 | - | expression tag |
His 47 | - | expression tag |
His 48 | - | expression tag |
His 49 | - | expression tag |
His 50 | - | expression tag |
Gly 51 | - | expression tag |
Ser 52 | - | expression tag |
Asp 59 | Asn 59 | engineered mutation |
Arg 308 | Lys 308 | engineered mutation |
Trp 533 | Cys 533 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 128236.0 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 670.1 | |
All* | Total formula weight | 128906.2 |