3MX1

The structure of GIY-YIG endonuclease R.Eco29kI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Eco29kIR	polymer	235	26793.0	1	UniProt (Q46944) Pfam (PF09517)	Escherichia coli	Restriction endonuclease
2	B (A)	water	water		18.0	170	Chemie (HOH)

Sequence modifications

A: 2 - 214 (UniProt: Q46944)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Gln 142	Glu 142	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26793.0
All*	Total formula weight	26793.0

*Water molecules are not included.