3MN9

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Actin-5C	polymer	374	41723.5	1	UniProt (P10987) Pfam (PF00022) In PDB	Drosophila melanogaster (Fruit fly)
2	X	Protein spire	polymer	19	1635.0	1	UniProt (Q9U1K1) In PDB	Drosophila melanogaster (Fruit fly)
3	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5		water	water		18.0	138	Chemie (HOH)

Sequence modifications

A: 2 - 375 (UniProt: P10987)

PDB	External Database	Details
Glu 204	Ala 205	engineered mutation
Lys 243	Pro 244	engineered mutation

X: 1 - 19 (UniProt: Q9U1K1)

PDB	External Database	Details
Unk 1	Gln 372	SEE REMARK 999
Unk 2	Val 373	SEE REMARK 999
Unk 3	Ile 374	SEE REMARK 999
Unk 4	Asp 375	SEE REMARK 999
Unk 5	Glu 376	SEE REMARK 999
Unk 6	Leu 377	SEE REMARK 999
Unk 7	Arg 378	SEE REMARK 999
Unk 8	Arg 379	SEE REMARK 999
Unk 9	Gly 380	SEE REMARK 999
Unk 10	Val 381	SEE REMARK 999
Unk 11	Arg 382	SEE REMARK 999
Unk 12	Leu 383	SEE REMARK 999
Unk 13	Lys 384	SEE REMARK 999
Unk 14	Lys 385	SEE REMARK 999
Unk 15	Ser 386	SEE REMARK 999
Unk 16	Asn 387	SEE REMARK 999
Unk 17	His 388	SEE REMARK 999
Unk 18	Glu 389	SEE REMARK 999
Unk 19	Arg 390	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43358.5
Non-Polymers*	Number of molecules	2
	Total formula weight	547.3
All*	Total formula weight	43905.8

*Water molecules are not included.