3MLQ

Crystal structure of the Thermus thermophilus transcription-repair coupling factor RNA polymerase interacting domain with the Thermus aquaticus RNA polymerase beta1 domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	DNA-directed RNA polymerase subunit beta	polymer	188	21081.1	4	UniProt (Q9KWU7) Pfam (PF04563)	Thermus aquaticus	RNAP subunit beta, Transcriptase subunit beta, RNA polymerase subunit beta
2	E, F, G, H (E, F, G, H)	Transcription-repair coupling factor	polymer	71	8002.2	4	UniProt (Q72KB4) Pfam (PF02559)	Thermus thermophilus
3	I (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)

Sequence modifications

A, B, C, D: 17 - 139 (UniProt: Q9KWU7)

PDB	External Database	Details
Met 16	-	initiating methionine

A, B, C, D: 334 - 395 (UniProt: Q9KWU7)

PDB	External Database	Details
Gly 332	-	linker
Gly 333	-	linker

E, F, G, H: 321 - 387 (UniProt: Q72KB4)

PDB	External Database	Details
Gly 317	-	expression tag
Pro 318	-	expression tag
His 319	-	expression tag
Met 320	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	116333.0
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	116428.0

*Water molecules are not included.