3MLG

2ouf-2x, a designed knotted protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	2X chimera of Helicobacter pylori protein HP0242	polymer	189	21962.0	2	UniProt (O25025) Pfam (PF09442) In PDB	Helicobacter pylori
2		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A, B: 1 - 92 (UniProt: O25025)
A, B: 101 - 181 (UniProt: O25025)

PDB	External Database	Details
Ser 93	-	linker
Gly 94	-	linker
Ser 95	-	linker
Gly 96	-	linker
Ser 97	-	linker
Gly 98	-	linker
Ser 99	-	linker
Ser 100	-	linker
Leu 182	-	expression tag
Glu 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43924.0
All*	Total formula weight	43924.0

*Water molecules are not included.