3MJ9

Crystal structure of JAML in complex with the stimulatory antibody HL4E10

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Junctional adhesion molecule-like	polymer	268	30647.4	1	UniProt (Q80UL9) Pfam (PF07686) In PDB	Mus musculus (mouse)	Dendritic cell-specific protein CREA7, mCrea7
2	L	STIMULATORY HAMSTER ANTIBODY HL4E10 FAB LIGHT CHAIN	polymer	213	22784.3	1		CRICETULUS MIGRATORIUS (ARMENIAN HAMSTER)
3	H	STIMULATORY HAMSTER ANTIBODY HL4E10 FAB HEAVY CHAIN	polymer	223	23653.5	1		CRICETULUS MIGRATORIUS (ARMENIAN HAMSTER)
4	B	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	1	In PDB GlyTouCan (G82348BZ)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A: 1 - 260 (UniProt: Q80UL9)

PDB	External Database	Details
Arg -1	-	expression tag
Ser 0	-	expression tag
Arg 124	Lys 144	engineered mutation
Gln 211	Arg 231	engineered mutation
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	77085.2
Branched	Number of molecules	1
	Total formula weight	1057.0
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	78584.6

*Water molecules are not included.