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3MFL

Axial Ligand Swapping In Double Mutant Maintains Intradiol-cleavage Chemistry in Protocatechuate 3,4-Dioxygenase

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, CProtocatechuate 3,4-dioxygenase alpha chainpolymer20022278.83UniProt (P00436)
Pfam (PF00775)
In PDB
Pseudomonas putida3,4-PCD
2M, N, OProtocatechuate 3,4-dioxygenase beta chainpolymer23826696.33UniProt (P00437)
Pfam (PF12391)
Pfam (PF00775)
In PDB
Pseudomonas putida3,4-PCD
3A, B, N, CSULFATE IONnon-polymer96.14Chemie (SO4)
4A, B, N, C, OGLYCEROLnon-polymer92.16Chemie (GOL)
5A, BBETA-MERCAPTOETHANOLnon-polymer78.13Chemie (BME)
6M, N, OFE (III) IONnon-polymer55.83Chemie (FE)
7MCARBONATE IONnon-polymer60.01Chemie (CO3)
8O, M, N2-(3,4-DIHYDROXYPHENYL)ACETIC ACIDnon-polymer168.13Chemie (DHY)
9OCHLORIDE IONnon-polymer35.51Chemie (CL)
10waterwater18.01048Chemie (HOH)
Sequence modifications
M, N, O: 301 - 538 (UniProt: P00437)
PDBExternal DatabaseDetails
His 447Tyr 148engineered mutation
Tyr 462His 163engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains6
Total formula weight146925.3
Non-Polymers*Number of molecules21
Total formula weight1938.7
All*Total formula weight148864.0
*Water molecules are not included.

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PDB entries from 2024-09-04

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