3MF4

Crystal structure of putative two-component system response regulator/ggdef domain protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Two-component system response regulator/GGDEF domain protein	polymer	259	29863.0	2	UniProt (A4SNL2)	Aeromonas salmonicida
2	C, D, E, F (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	G, H (A, B)	water	water		18.0	249	Chemie (HOH)

Sequence modifications

A, B: 2 - 249 (UniProt: A4SNL2)

PDB	External Database	Details
Mse -1	-	expression tag
Ser 0	-	expression tag
Leu 1	-	expression tag
Mse 17	Thr 17	engineered mutation
Gln 148	Leu 148	engineered mutation
Ala 160	Thr 160	engineered mutation
His 165	Asn 165	engineered mutation
Glu 250	-	expression tag
Gly 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59726.0
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	59823.3

*Water molecules are not included.