3M8U

Crystal structure of glutathione-binding protein A (GbpA) from Haemophilus parasuis SH0165 in complex with glutathione disulfide (GSSG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Heme-binding protein A	polymer	522	58651.8	1	UniProt (B8F653) Pfam (PF00496) In PDB	Haemophilus parasuis
2	A	OXIDIZED GLUTATHIONE DISULFIDE	non-polymer		612.6	1	Chemie (GDS)
3	A	MALONATE ION	non-polymer		102.0	3	Chemie (MLI)
4		water	water		18.0	594	Chemie (HOH)

Sequence modifications

A: 24 - 531 (UniProt: B8F653)

PDB	External Database	Details
Met 23	-	expression tag
Ser 145	Pro 145	engineered mutation
Met 156	Asp 156	engineered mutation
Leu 210	Val 210	engineered mutation
Leu 223	Phe 223	engineered mutation
His 281	Leu 281	engineered mutation
Ser 351	Asn 351	engineered mutation
Lys 532	-	expression tag
Leu 533	-	expression tag
Ala 534	-	expression tag
Ala 535	-	expression tag
Ala 536	-	expression tag
Leu 537	-	expression tag
Glu 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58651.8
Non-Polymers*	Number of molecules	4
	Total formula weight	918.8
All*	Total formula weight	59570.6

*Water molecules are not included.