3M62

Crystal structure of Ufd2 in complex with the ubiquitin-like (UBL) domain of Rad23

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin conjugation factor E4	polymer	968	110806.3	1	UniProt (P54860) Pfam (PF10408) Pfam (PF04564)	Saccharomyces cerevisiae (yeast)	Ubiquitin fusion degradation protein 2, UB fusion protein 2
2	B (B)	UV excision repair protein RAD23	polymer	106	11885.6	1	UniProt (P32628) Pfam (PF00240)	Saccharomyces cerevisiae (yeast)
3	C (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
4	D (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	E, F (A, B)	water	water		18.0	298	Chemie (HOH)

Sequence modifications

A: 1 - 961 (UniProt: P54860)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Pro -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag
Leu 102	Ser 102	engineered mutation
Val 677	Asp 677	engineered mutation

B: 1 - 84 (UniProt: P32628)

PDB	External Database	Details
Arg 85	-	expression tag
Asp 86	-	expression tag
Pro 87	-	expression tag
Asn 88	-	expression tag
Ser 89	-	expression tag
Ser 90	-	expression tag
Ser 91	-	expression tag
Val 92	-	expression tag
Asp 93	-	expression tag
Lys 94	-	expression tag
Leu 95	-	expression tag
Ala 96	-	expression tag
Ala 97	-	expression tag
Ala 98	-	expression tag
Leu 99	-	expression tag
Glu 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
His 106	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	122691.9
Non-Polymers*	Number of molecules	2
	Total formula weight	277.4
All*	Total formula weight	122969.3

*Water molecules are not included.